Lipocalin 2 Protein (LCN2) (His tag)

Catalog No. ABIN7318827

Recombinant Lipocalin 2 protein expressed in Escherichia coli (E. coli).

Quick Overview for Lipocalin 2 Protein (LCN2) (His tag) (ABIN7318827)

Target: Lipocalin 2 (LCN2)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Lipocalin 2 protein is labelled with His tag.

Purpose: Recombinant Human NGAL/Lipocalin-2 Protein (His Tag, E.coli)

Sequence: Gln21-Gly198

Characteristics: Recombinant Human Neutrophil gelatinase-associated lipocalin is produced by our E.coli expression system and the target gene encoding Gln21-Gly198 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS,50 % glycerol, pH 7.4.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: Lipocalin 2 (LCN2)

Alternative Name: NGAL/Lipocalin-2

Background:

Background: Neutrophil gelatinase-associated lipocalin(LCN2) is a secreted protein and belongs to the calycin superfamily. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI). Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein which specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect, as has already been observed in different tumors (e.g. breast, stomach, oesophagus, brain) in humans. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival and morphogenesis.

Synonym: Neutrophil gelatinase-associated lipocalin, NGAL, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25, HNL, NGAL

Molecular Weight: 21.8 kDa

UniProt: P80188

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis