MUC16 Protein (His tag)

Catalog No. ABIN7319764

This Recombinant MUC16 protein is produced in Human Cells.

Quick Overview for MUC16 Protein (His tag) (ABIN7319764)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: >95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This MUC16 protein is labelled with His tag.

Purpose: Recombinant Human CA125/MUC16 (C-6His)

Sequence: Gly12660-Met12923

Characteristics: Recombinant Human Mucin-16 is produced by our Mammalian expression system and the target gene encoding Gly12660-Met12923 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human Mesothelin(Cat#CP52) at 10μg/ml (100 μl/well) can bind Human CA125-His (Cat#CW57).The ED50 of Human CA125-His (Cat#CW57) is 0.6μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 100 mM Glycine, pH 7.5.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: CA125/MUC16

Background:

Background: MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. MUC16 is over-expressed by tumor cells including ovarian and mesothelial cancers. The transmembrane form can adhere to mesothelin in the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity. MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells. MUC16-expressing tumors adhere to NK cells, down-regulate CD16 and suppress NK response, which may promote immune evasion. MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy. In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome.

Synonym: CA125 ovarian cancer antigen, CA125, CA-125, CA125MUC-16, FLJ14303, MUC16, mucin 16, cell surface associated, mucin-16

Molecular Weight: 29.2 kDa

UniProt: Q8WXI7