Natural Cytotoxicity Triggering Receptor 3 Ligand 1 (NCR3LG1) protein (His tag)

Catalog No. ABIN7197101

Recombinant Natural Cytotoxicity Triggering Receptor 3 Ligand 1 protein expressed in HEK-293 Cells.

Quick Overview for Natural Cytotoxicity Triggering Receptor 3 Ligand 1 (NCR3LG1) protein (His tag) (ABIN7197101)

Target: Natural Cytotoxicity Triggering Receptor 3 Ligand 1 (NCR3LG1)

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Rat B7-H6/NCR3LG1 Protein (His Tag)

Sequence: Met1-Ser308

Characteristics: A DNA sequence encoding the rat NCR3LG1 (XP_006223356.1) (Met1-Ser308) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Natural Cytotoxicity Triggering Receptor 3 Ligand 1 (NCR3LG1)

Alternative Name: B7-H6/NCR3LG1

Background:

Background: Natural cytotoxicity triggering receptor 3 ligand 1(B7-H6) is a glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors.

Synonym: B7H6

Molecular Weight: 35.1 kDa

NCBI Accession: XP_006223356