NCALD Protein (His tag)

Catalog No. ABIN7318816

This Recombinant NCALD protein is expressed in Escherichia coli (E. coli).

Quick Overview for NCALD Protein (His tag) (ABIN7318816)

Target: NCALD (Neurocalcin delta (NCALD))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This NCALD protein is labelled with His tag.

Purpose: Recombinant Human Neurocalcin-δ/NCALD Protein (His Tag)

Sequence: Met 1-Phe193

Characteristics: Recombinant Human Neurocalcin-delta is produced by our E.coli expression system and the target gene encoding Met1-Phe193 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 40 % Glycerol, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: NCALD (Neurocalcin delta (NCALD))

Alternative Name: Neurocalcin-delta/NCALD

Background:

Background: Neurocalcin-delta (NCALD) is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family. It expressed in mammalian brains. NCALD contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein possesses a Ca2+ /myristoyl switch. It is cytosolic at resting calcium levels. However, elevated intracellular calcium levels induce a conformational change which exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is thought to be a regulator of G protein-coupled receptor signal transduction.

Synonym: Neurocalcin-Delta, NCALD

Molecular Weight: 24.4 kDa

UniProt: P61601