NCALD Protein (AA 1-193) (His tag)
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- Target See all NCALD Proteins
- NCALD (Neurocalcin delta (NCALD))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-193
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NCALD protein is labelled with His tag.
- Purpose
- Recombinant Human Neurocalcin-δ/NCALD (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF
- Characteristics
- Recombinant Human Neurocalcin-D/NCALD is produced by our E. coli expression system. The target protein is expressed with sequence (Gly2-Phe193) of Human NCALD fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product NCALD Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 40 % Glycerol, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- NCALD (Neurocalcin delta (NCALD))
- Alternative Name
- Neurocalcin-delta (NCALD Products)
- Synonyms
- AI848120 Protein, D030020D09Rik Protein, D15Ertd412e Protein, nca Protein, NCALD Protein, zgc:175182 Protein, ncald Protein, zgc:100982 Protein, neurocalcin delta Protein, neurocalcin delta L homeolog Protein, Neurocalcin delta Protein, neurocalcin delta a Protein, neurocalcin delta b Protein, Ncald Protein, NCALD Protein, ncald.L Protein, Bm1_53910 Protein, LOAG_16127 Protein, ncald Protein, ncalda Protein, ncaldb Protein
- Sub Type
- Fusionprotein
- Background
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Neurocalcin-delta (NCALD) is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family. It expressed in mammalian brains. NCALD contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein possesses a Ca2+ /myristoyl switch. It is cytosolic at resting calcium levels. However, elevated intracellular calcium levels induce a conformational change which exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is thought to be a regulator of G protein-coupled receptor signal transduction.
Alternative Names: Neurocalcin-Delta, NCALD - Molecular Weight
- 24.4 kDa
- UniProt
- P61601
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