NIP7 Protein (His tag)
Quick Overview for NIP7 Protein (His tag) (ABIN7318829)
Target
See all NIP7 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This NIP7 protein is labelled with His tag.
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Purpose
- Recombinant Human NIP7/KD93 Protein (His Tag)
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Sequence
- Met 1-Thr180
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Characteristics
- Recombinant Human 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Thr180 is expressed with a 6His tag at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Nuclear Import 7 Homolog (NIP7) (AA 1-180) protein (His tag)
custom-made NIP7 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nuclear Import 7 Homolog (NIP7) (AA 1-180) protein (His tag)custom-made NIP7 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nuclear Import 7 Homolog (NIP7) (AA 1-180) protein (GST tag)NIP7 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nuclear Import 7 Homolog (NIP7) (AA 1-180) protein (His-IF2DI Tag)NIP7 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Nuclear Import 7 Homolog (NIP7) (AA 1-180), (full length) protein (His-SUMO Tag)NIP7 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Nuclear Import 7 Homolog (NIP7) protein (Myc-DYKDDDDK Tag)NIP7 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0 .
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- NIP7 (Nuclear Import 7 Homolog (NIP7))
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Alternative Name
- NIP7/KD93
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Background
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Background: 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog (NIP7) belongs to the NIP7 family. NIP7 contains one PUA domain, it is essential for the process of proper 27S pre-rRNA and 60S ribosome subunit assembly. NIP7 is a monomer form and interacts with NOL8 and SBDS, and may bind to RNA. In addition, NIP7 is one of the many trans-acting factors required for eukaryotic ribosome biogenesis, which interacts with nascent pre-ribosomal particles and dissociates as they complete maturation and are exported to the cytoplasm.
Synonym: 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog, KD93, NIP7
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Molecular Weight
- 9.0 kDa
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UniProt
- Q9Y221
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Pathways
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
Target
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