Pepsinogen 4, Group I (Pepsinogen A) L Homeolog (PGA4.L) (Active) protein (His tag)

Catalog No. ABIN7197291

This Recombinant Pepsinogen 4, Group I (Pepsinogen A) L Homeolog protein is produced in HEK-293 Cells.

Quick Overview for Pepsinogen 4, Group I (Pepsinogen A) L Homeolog (PGA4.L) (Active) protein (His tag) (ABIN7197291)

Target: Pepsinogen 4, Group I (Pepsinogen A) L Homeolog (PGA4.L)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 97 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Human PGA4/Pepsinogen A Protein (His Tag)(Active)

Sequence: Met 1-Ala 388

Characteristics: A DNA sequence encoding the human PGA4 (NP_001073276.1) (Met 1-Ala 388) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114.The specific activity is > 10,000 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH)

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Pepsinogen 4, Group I (Pepsinogen A) L Homeolog (PGA4.L)

Alternative Name: PGA4/Pepsinogen A

Background:

Background: PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.

Synonym: FLJ58952,FLJ77962,PGA3,PGA4,PGA5

Molecular Weight: 41.7 kDa

UniProt: P00790