PPCDC Protein (His tag)
Quick Overview for PPCDC Protein (His tag) (ABIN7318902)
Target
See all PPCDC ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This PPCDC protein is labelled with His tag.
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Purpose
- Recombinant Human PPCDC Protein (His Tag)
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Sequence
- Met 1-Ser204
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Characteristics
- Recombinant Human Phosphopantothenoylcysteine Decarboxylase is produced by our E.coli expression system and the target gene encoding Met1-Ser204 is expressed with a 6His tag at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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phosphopantothenoylcysteine Decarboxylase (PPCDC) (AA 1-204) protein (His tag)
custom-made PPCDC Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
phosphopantothenoylcysteine Decarboxylase (PPCDC) (AA 1-204) protein (His tag)custom-made PPCDC Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
phosphopantothenoylcysteine Decarboxylase (PPCDC) (AA 1-204) protein (His-IF2DI Tag)PPCDC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
phosphopantothenoylcysteine Decarboxylase (PPCDC) (AA 1-204) protein (GST tag)PPCDC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 50 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 8.0.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- PPCDC (phosphopantothenoylcysteine Decarboxylase (PPCDC))
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Alternative Name
- PPCDC
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Background
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Background: Phosphopantothenoylcysteine Decarboxylase (PPC-DC) is an essential enzyme in the biosynthesis ofCoenzyme A and catalyzes the decarboxylation of PPC to Phosphopantetheine. PPC-DC catalyzes the decarboxylation of the Cysteine moiety of 4-Phosphopantothenoylcysteine (PPC) to form 4-Phosphopantetheine (PPantSH), this reaction forms part of the biosynthesis of Coenzyme A. The enzyme is a member of the larger family of Cysteine Decarboxylases including the Lantibiotic-Biosynthesizing enzymes EpiD and MrsD, all of which use a tightly bound Flavin cofactor to oxidize the Thiol moiety of the substrate to a Thioaldehyde.
Synonym: Phosphopantothenoylcysteine Decarboxylase, PPC-DC, PPCDC, COAC
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Molecular Weight
- 24.6 kDa
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UniProt
- Q96CD2
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Pathways
- Ribonucleoside Biosynthetic Process
Target
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