PDGFRB Protein (His tag)

Catalog No. ABIN7197403

This Recombinant PDGFRB protein is expressed in HEK-293 Cells.

Quick Overview for PDGFRB Protein (His tag) (ABIN7197403)

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This PDGFRB protein is labelled with His tag.

Purpose: Recombinant Rat PDGFRB/CD140b Protein (His Tag)(Active)

Sequence: Met1-Lys530

Characteristics: A DNA sequence encoding the rat PDGFRB (Q05030) (Met1-Lys530) was expressed, fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized rat PDGFRB-His at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB , The EC50 of Cynomolgus PDGFB is 3-7 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: PDGFRB/CD140b

Background:

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: PDGFRB;Pdgfr;Pdgfr1;PDGFR-1

Molecular Weight: 57.6 kDa

UniProt: Q05030

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling