PRDM2 Protein

Catalog No. ABIN7317619

Recombinant PRDM2 protein expressed in Escherichia coli (E. coli).

Quick Overview for PRDM2 Protein (ABIN7317619)

Target: PRDM2 (PR Domain Containing 2, with ZNF Domain (PRDM2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 96 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human PRDM2/RIZ1 Protein

Sequence: Met 1-Ala 200

Characteristics: A DNA sequence encoding the N-terminal segment of human PRDM2 (NP_036363.2) (Met 1-Ala 200), containing the SET domian, was expressed, with two additonal amino acids (Gly & Pro) at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 0.5 mM DTT, 0.5 mM GSH, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PRDM2 (PR Domain Containing 2, with ZNF Domain (PRDM2))

Alternative Name: PRDM2/RIZ1

Background:

Background: PR domain containing 2, with ZNF domain (PRDM2), also known as zinc finger protein RIZ, is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain, which shares sequence homology with an independently described sequence motif, the PR domain. PRDM2 contains 8 C2H2-type zinc fingers and a distinct SET domain, and is highly expressed in retinoblastoma cell lines and in brain tumors, as well as in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. PRDM2 is a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3, and is identified as a tumor suppressor. It is reported that intact PR( SET) sequence is required for tumor suppression functions, mutations in the PR domain caused activity reduction in human cancers. Also, S-adenosylhomocysteine or methyl donor deficiency inhibits RIZ1 and other H3 lysine 9 methylation activities. PRDM2 may also function as a DNA-binding transcription factor. It Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and act as a transcriptional activator. In addition, PRDM2 (RIZ) is able to binds to the retinoblastoma protein (RB) and also Interacts with GATA3.

Synonym: HUMHOXY1,KMT8,MTB-ZF,RIZ,RIZ1,RIZ2

Molecular Weight: 23 kDa

NCBI Accession: NP_036363