PRSS2 Protein (His tag)

Catalog No. ABIN7197544

This Recombinant PRSS2 protein is expressed in HEK-293 Cells.

Quick Overview for PRSS2 Protein (His tag) (ABIN7197544)

Target: PRSS2 (Protease, serine, 2 (Trypsin 2) (PRSS2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 92 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This PRSS2 protein is labelled with His tag.

Purpose: Recombinant Mouse Trypsin 2/PRSS2 Protein (His Tag)(Active)

Sequence: Met 1-Asn 246

Characteristics: A DNA sequence encoding the mouse PRSS2 (NP_033456.1) precursor (Met 1-Asn 246) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog #27096). The specific activity is >1500 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PRSS2 (Protease, serine, 2 (Trypsin 2) (PRSS2))

Alternative Name: Trypsin 2/PRSS2

Background:

Background: Trypsin-2, also known as Trypsin II, Anionic trypsinogen, Serine protease 2, PRSS2 and TRY2, is a secreted protein which belongs to the trypsin serine protease family including Trypsin, PRSS1, PRSS2 and PRSS3. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a proteolytically active mature chain (residues 24-247). PRSS2 contains one peptidase S1 domain. It is secreted into the duodenum, hydrolysing peptides into their smaller building blocks, which is necessary for the uptake of protein in the food. It is secreted by the pancreas in the form of inactive zymogen, trypsinogen and cleaved to its active form in the small intestine when the pancreas is stimulated by cholecystokinin through the common activation mechanism.

Synonym: Prss2;Ta;Tesp4;Try2;TRY8;TRYP

Molecular Weight: 26.2 kDa

NCBI Accession: NP_033456