PRKD3 Protein (GST tag)
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- Target See all PRKD3 Proteins
- PRKD3 (Protein Kinase D3 (PRKD3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PRKD3 protein is labelled with GST tag.
- Purpose
- Recombinant Human PKC nu/PRKD3 Protein (GST Tag)
- Sequence
- Met 1-Pro 890
- Characteristics
- A DNA sequence encoding the full length of human PRKD3 (NP_005804.1) (Met 1-Pro 890) was expressed with the GST tag at the N-terminus.
- Purity
- > 85 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product PRKD3 Protein
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Protein Kinase D3 (PRKD3) (AA 3-890) (Active) protein
PRKD3 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Protein Kinase D3 (PRKD3) (AA 1-100) protein (GST tag)PRKD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase D3 (PRKD3) (AA 1-890) protein (Strep Tag)Crystallography grade PRKD3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein Kinase D3 (PRKD3) (AA 1-889) protein (Strep Tag)Crystallography grade PRKD3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 mM Reduced Glutathione, pH 7.4
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- PRKD3 (Protein Kinase D3 (PRKD3))
- Alternative Name
- PKC nu/PRKD3 (PRKD3 Products)
- Synonyms
- EPK2 Protein, PKC-NU Protein, PKD3 Protein, PRKCN Protein, nPKC-NU Protein, PRKD3 Protein, 4930557O20Rik Protein, 5730497N19Rik Protein, Pkcnu Protein, Prkcn Protein, protein kinase D3 Protein, PRKD3 Protein, prkd3 Protein, Prkd3 Protein
- Background
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Background: Serine/threonine-protein kinase D3, also known as Protein kinase C nu type, Protein kinase EPK2, PRKD3, EPK2 and PRKCN, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD3 / PRKCN contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PRKD3 / PRKCN converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. It is involved in resistance to oxidative stress. PRKD3 / PRKCN is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735 and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain. PRKD3 / PRKCN can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. PRKD3 / PRKCN can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phospholipase C gamma and the involvement of other PKC family members.
Synonym: EPK2;nPKC-NU;PKC-NU;PKD3;PRKCN
- Molecular Weight
- 126.7 kDa
- NCBI Accession
- NP_005804
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