RSPO1 Protein (His tag)

Catalog No. ABIN7320321

Recombinant RSPO1 protein expressed in CHO Cells.

Quick Overview for RSPO1 Protein (His tag) (ABIN7320321)

Target: RSPO1 (R-Spondin 1 (RSPO1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: CHO Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This RSPO1 protein is labelled with His tag.

Purpose: Recombinant Mouse R-Spondin 1/RSPO1 Protein (His Tag)(Active)

Sequence: Met 1-Gln 265

Characteristics: A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: RSPO1 (R-Spondin 1 (RSPO1))

Alternative Name: R-Spondin 1/RSPO1

Background:

Background: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

Synonym: R-spondin,Rspondin

Molecular Weight: 28.5 kDa

NCBI Accession: NP_619624