Reticulon 4 Protein (RTN4) (GST tag)

Catalog No. ABIN7317322

This Recombinant Reticulon 4 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Reticulon 4 Protein (RTN4) (GST tag) (ABIN7317322)

Target: Reticulon 4 (RTN4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 92 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Reticulon 4 protein is labelled with GST tag.

Purpose: Recombinant Human RTN4/NOGO-A Protein (GST Tag)(Active)

Sequence: Met 1-Val 185

Characteristics: A DNA sequence encoding the human RTN4 (NP_065393.1) N-terminal fragment (Met 1-Val 185) was fused with the GST tag at the N-terminus.

Biological Activity Comment: Measured by its ability to bind recombinant human RTN4R in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 1 mM DTT, 0.2 mM GSH, pH 7.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Reticulon 4 (RTN4)

Alternative Name: RTN4/NOGO-A

Background:

Background: Reticulon-4, also known as Foocen, Neurite outgrowth inhibitor, Nogo protein, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, RTN-x, Reticulon-5 and RTN4, is a multi-pass membrane protein which contains one reticulon domain. Isoform 1 of RTN4 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 of RTN4 is widely expressed except for the liver. Isoform 3 of RTN4 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 of RTN4 is testis-specific. Reticulon-4 / RTN4 is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon-4 / RTN4 regulates neurite fasciculation, branching and extension in the developing nervous system. Reticulon-4 / RTN4 is involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 of RTN4 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 of RTN4 inhibit BACE1 activity and amyloid precursor protein processing.

Synonym: ASY;DGU;DKFZp781L1143;HIGM4;Nbla00271;Nbla10545;NI220/250;NOGO;NOGO-A;Nogo-B;Nogo-C;NOGOC;NSP;NSP-CL;RTN-X;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;UDG;UNG1;UNG15;UNG2

Molecular Weight: 46.2 kDa

NCBI Accession: NP_065393

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome