Riboflavin Kinase Protein (RFK) (His tag)
Quick Overview for Riboflavin Kinase Protein (RFK) (His tag) (ABIN7197718)
Target
See all Riboflavin Kinase (RFK) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This Riboflavin Kinase protein is labelled with His tag.
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Purpose
- Recombinant Human Flavokinase/RFK Protein (His Tag)
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Sequence
- Met 1-His 155
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Characteristics
- A DNA sequence encoding the mature form of human RFK (Q969G6) (Met1-His155) was expressed with a polyhistide tag at the N-terminus.
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Riboflavin Kinase (RFK) (AA 1-155) protein (His tag)
custom-made RFK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Riboflavin Kinase (RFK) (AA 1-155) protein (His tag)custom-made RFK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Riboflavin Kinase (RFK) (AA 1-162) protein (GST tag)RFK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Riboflavin Kinase (RFK) (AA 1-162) protein (His tag)RFK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Riboflavin Kinase (RFK) protein (Myc-DYKDDDDK Tag)RFK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as sterile 20 mM Tris, 10 % glycerol, pH 8.0
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Riboflavin Kinase (RFK)
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Alternative Name
- Flavokinase/RFK
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Background
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Background: Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.
Synonym: RIFK
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Molecular Weight
- 19.5 kDa
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UniProt
- Q969G6
Target
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