Sclerostin Protein (SOST) (His tag)

Catalog No. ABIN7320639

This Recombinant Sclerostin protein is produced in Human Cells.

Quick Overview for Sclerostin Protein (SOST) (His tag) (ABIN7320639)

Target: Sclerostin (SOST)

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Purity: > 90 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This Sclerostin protein is labelled with His tag.

Purpose: Recombinant Mouse Sclerostin/SOST Protein (His Tag)

Sequence: Gln24-Tyr211

Characteristics: Recombinant Mouse Sclerostin is produced by our Mammalian expression system and the target gene encoding Gln24-Tyr211 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Sclerostin (SOST)

Alternative Name: Sclerostin/SOST

Background:

Background: Sclerostin (SOST) is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization? however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity.

Synonym: Sclerostin, Sost

Molecular Weight: 21.9 kDa

UniProt: Q99P68