SEMA4D/CD100 Protein (His tag)
Quick Overview for SEMA4D/CD100 Protein (His tag) (ABIN7321133)
Target
See all SEMA4D/CD100 (SEMA4D) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This SEMA4D/CD100 protein is labelled with His tag.
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Purpose
- Recombinant Rat Semaphorin 4D/SEMA4D/ Protein (His Tag)
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Sequence
- Met1-Met712
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Characteristics
- A DNA sequence encoding the rat SEMA4D (Met1-Met712) was expressed, fused with a polyhistidine tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method
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Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 22-734) protein (His-Avi Tag,Biotin)
SEMA4D Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 24-734) protein (His tag)SEMA4D Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 22-734) protein (His-Avi Tag)SEMA4D Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 24-733) protein (His tag)SEMA4D Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) protein (Fc Tag)SEMA4D Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 759-862) protein (His-IF2DI Tag)SEMA4D Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 22-734) protein (His tag)SEMA4D Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) protein (Fc Tag)SEMA4D Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (Active) protein (His tag)SEMA4D Origin: Mouse Host: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile PBS, pH 7.4
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
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Alternative Name
- Semaphorin 4D/SEMA4D
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Background
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Background: Semaphorin-4D is also known as A8,BB18, GR3, CD100. Semaphorin-4D belongs to the semaphorin family containing 1 Ig-like C2-type domain, 1 PSI domain and 1 Sema domain. It is the cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. It promotes the migration of cerebellar granule cells and of endothelial cells, regulates dendrite and axon branching and morphogenesis. Semaphorin-4D Plays a role in the immune system, Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade.
Synonym: SEMA4D
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Molecular Weight
- 77.8 kDa
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NCBI Accession
- NP_001164034
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Pathways
- Regulation of Cell Size
Target
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