SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag,Biotin)
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- Target See all SEMA4D/CD100 (SEMA4D) Proteins
- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-734
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SEMA4D/CD100 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein
- Sequence
- Met22-Arg734
- Characteristics
- Recombinant Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-Semaphorin 4D Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Semaphorin 4D, His Tag with the EC50 of 44.8ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
- Alternative Name
- Semaphorin 4D (SEMA4D Products)
- Synonyms
- C9orf164 Protein, CD100 Protein, M-sema-G Protein, SEMAJ Protein, coll-4 Protein, Semacl2 Protein, Semaj Protein, Semcl2 Protein, COLL-4 Protein, semaphorin 4D Protein, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D Protein, Sema4d Protein, SEMA4D Protein
- Background
- Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.
- Molecular Weight
- 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Cell Size
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