CD43 Protein (Fc Tag)

Catalog No. ABIN7320163

Recombinant CD43 protein expressed in HEK-293 Cells.

Quick Overview for CD43 Protein (Fc Tag) (ABIN7320163)

Target: CD43 (SPN) (Sialophorin (SPN))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 80 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CD43 protein is labelled with Fc Tag.

Purpose: Recombinant Mouse SPN/CD43 Protein (Fc Tag)

Sequence: Met 1-Gly 248

Characteristics: A DNA sequence encoding the extracellular domain of mouse SPN (P15702) (Met 1-Gly 248) was fused with the Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: SPN/CD43

Background:

Background: CD43 is an abundantly expressed molecule on the T-cell surface that shows distinct localization to the migrating T-cell uropod and the distal pole complex (DPC) opposite the immunological synapse via association with the ezrin-radixin-moesin (ERM) family of actin regulatory proteins. CD43 has a 235-amino acid (aa) extracellular domain, a 23-aa transmembrane domain, and a 123-aa cytoplasmic domain, all encoded by a single exon. The intracytoplasmic region of the protein is necessary to transduce signals, it is rich in potentially phosphorylable threonines and serines but lacks tyrosine residues as well as catalytic activity. CD43 engagement on human peripheral blood T cells and monocytes leads to cell activation and proliferation through the generation of second messengers such as diacylglycerol and inositol phosphates, protein kinase C (PKC) activation and Ca2+ mobilization. In addition, CD43 ligation on human T cells induces the association of CD43 with Src family kinases, presumably through the interaction of their Src homology 3 domain with a proline-rich region of the CD43 intracytoplasmic tail. This molecule has been implicated in T cell activation, enhancing T cell response to allogeneic or mitogenic stimulation and CD43-specific signals have been reported to be sufficient to activate T cells in the absence of T cell receptor (TCR) engagement. In summary, CD43 regulates multiple T-cell functions, including T-cell activation, proliferation, apoptosis, and migration.

Synonym: A630014B01Rik,Cd43,Galgp,Ly-48,Ly48

Molecular Weight: 49.6 kDa

UniProt: P15702

Pathways: Regulation of Leukocyte Mediated Immunity