TIMD4 Protein (His tag)

Catalog No. ABIN7320296

Recombinant TIMD4 protein expressed in HEK-293 Cells.

Quick Overview for TIMD4 Protein (His tag) (ABIN7320296)

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 94 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This TIMD4 protein is labelled with His tag.

Purpose: Recombinant Mouse TIM4/TIMD4 Protein (His Tag)

Sequence: Met 1-Thr 279

Characteristics: A DNA sequence encoding the extracellular domain of mouse TIMD4 (NP_848874.3) precursor (Met 1-Thr 279) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Alternative Name: TIM4/TIMD4

Background:

Background: A type I transmembrane protein called TIM4 (T-cell immunoglobulin- and mucin-domain-containing molecule, also known as TIMD4), which belongs to the immunoglobulin superfamily and TIM family. TIM4 is involved in regulating T-cell proliferation and lymphotoxin signaling. It is a ligand for HAVCR1/TIMD1. Recent reports indicate that dendritic cell (DC)-derived T-cell immunoglobulin and mucin domain molecule (TIM)-4, which is expressed on dendritic cells and macrophages, plays an important role in the initiation of T(H)2 polarization. TIM4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. The expression of TIM4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIM4 is phosphatidylserine receptor for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved. Modulation of TIM4 production in dendritic cells (DCs) represents a novel therapeutic approach for the treatment of peanut allergy. The interaction of TIM1/TIM4 played a critical role in sustaining the polarization status of Th2 cells in allergic rhinitis (AR) patients. Cross-linking FcgammaRI by antigen/IgG complexes increased the production of TIM4 by dendritic cells via upregulating tumor necrosis factor-alpha in DCs. Specific immunotherapy (SIT) suppresses the skewed Th2 responses via disrupting the interaction of TIM1/TIM4 in antigen-specific Th2 cells.

Synonym: B430010N18Rik,TIM-4,Tim4

Molecular Weight: 29.3 kDa

NCBI Accession: NP_848874

Pathways: Cancer Immune Checkpoints