Thyroperoxidase Protein (His tag)

Catalog No. ABIN7198245

This Recombinant Thyroperoxidase protein is produced in Baculovirus infected Insect Cells.

Quick Overview for Thyroperoxidase Protein (His tag) (ABIN7198245)

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Thyroperoxidase protein is labelled with His tag.

Purpose: Recombinant Human Thyroid peroxidase/TPO Protein (257 Ser/Ala, 725 Pro/Thr, His Tag)

Sequence: Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr

Characteristics: A DNA sequence encoding the human TPO (P07202-1) extracellular domain (Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Alternative Name: Thyroid peroxidase/TPO

Background:

Background: Thyroid peroxidase is a membrane-bound glycoprotein which belongs to the peroxidase family, XPO subfamily. It contains 1 EGF-like domain and 1 Sushi (CCP/SCR) domain. Thyroid Peroxidase represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. It used to be taken as the formerly so-called `microsomal antigen` several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx 100 kDa molecular weight. Thyroid peroxidase is an enzyme expressed abundantly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine or triiodothyronine, thyroid hormones. Thyroid peroxidase plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin to form precursors of the thyroid hormones T4 and T3.

Synonym: MSA,TDH2A,TPX

Molecular Weight: 93.8 kDa

Pathways: Thyroid Hormone Synthesis