UBE2G1 Protein

Catalog No. ABIN7198583

Recombinant UBE2G1 protein expressed in Escherichia coli (E. coli).

Quick Overview for UBE2G1 Protein (ABIN7198583)

Target: UBE2G1 (Ube2g1) (Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human UBE2G1 Protein

Sequence: Met 1-Glu 170

Characteristics: A DNA sequence encoding the human UBE2G1 (P62253) (Met 1-Glu 170) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 10 % glycerol, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: UBE2G1 (Ube2g1) (Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1))

Alternative Name: UBE2G1

Background:

Background: UBE2G1 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 

Synonym: E217K;UBC7;UBE2G

Molecular Weight: 19.5 kDa

UniProt: P62253