UBE2M Protein

Catalog No. ABIN7198593

This Recombinant UBE2M protein is produced in Escherichia coli (E. coli).

Quick Overview for UBE2M Protein (ABIN7198593)

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human UBE2M/UBC12 Protein

Sequence: Met 1-Lys 183

Characteristics: A DNA sequence encoding the human UBE2M (P61081) (Met 1-Lys 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 10 % glycerol, 1 mM DTT, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBE2M/UBC12

Background:

Background: UBE2M is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 

Synonym: NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M; UBC12; UBE2M;

Molecular Weight: 21 kDa

UniProt: P61081