UNG Protein (GST tag)

Catalog No. ABIN7317329

This Recombinant UNG protein is produced in Escherichia coli (E. coli).

Quick Overview for UNG Protein (GST tag) (ABIN7317329)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This UNG protein is labelled with GST tag.

Purpose: Recombinant Human Uracil-DNA glycosylase/UNG Protein (GST Tag)

Sequence: Phe 85-Leu 304

Characteristics: A DNA sequence encoding the human UNG isoform 1 (P13051-2) (Phe 85-Leu 304) was fused with the GST tag at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 40 mM Tris, 0.15M NaCl, 2 mM GSH, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: UNG (Uracil-DNA Glycosylase (UNG))

Alternative Name: Uracil-DNA glycosylase/UNG

Target Type: Viral Protein

Background:

Background: Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells. Uracil-DNA glycosylase exists in two forms: mitochondrial uracil-DNA glycosylase 1 (UNG1) and nuclear uracil-DNA glycosylase 2 (UNG2). uracil-DNA glycosylase. This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections.

Synonym: DGU,HIGM4,HIGM5,UDG,UNG1,UNG15,UNG2

Molecular Weight: 52 kDa

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response