VEGF-165 / VEGF-A (Active) Protein

Catalog No. ABIN7319413

This Recombinant VEGF-165 / VEGF-A protein is expressed in Human Cells.

Quick Overview for VEGF-165 / VEGF-A (Active) Protein (ABIN7319413)

Target: VEGF-165 / VEGF-A

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human VEGF165/VEGFA Protein (Active)

Sequence: Ala27-Arg191

Characteristics: Recombinant Human VEGF165 is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human VEGF165 at 10μg/ml (100 μl/well) can bind Human VEGFR2, The ED50 of Human VEGF165 is 433.6 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: VEGF-165 / VEGF-A

Alternative Name: VEGF165/VEGFA

Background:

Background: Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

Synonym: MVCD1,VEGF,VEGF165,VPF,Vascular Endothelial Growth Factor Isoform 165

Molecular Weight: 19.1 kDa