Vinculin Protein (VCL) (His tag)
Quick Overview for Vinculin Protein (VCL) (His tag) (ABIN7198696)
Target
See all Vinculin (VCL) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This Vinculin protein is labelled with His tag.
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Purpose
- Recombinant Mouse VCL/Vinculin Protein (His Tag)
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Sequence
- Met1-Gln1066
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Characteristics
- A DNA sequence encoding the mouse VCL (NP_033528.3) (Met1-Gln1066) was expressed with a C-terminal polyhistidine tag.
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Vinculin (VCL) (AA 2-235) protein (His tag)
VCL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vinculin (VCL) (AA 777-1066) protein (GST tag)VCL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Vinculin (VCL) (AA 1-1066) protein (GST tag)VCL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Vinculin (VCL) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)VCL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile PBS, pH 7.4
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Vinculin (VCL)
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Alternative Name
- VCL/Vinculin
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Background
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Background: Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin.
Synonym: 9430097D22,AA571387,AI462105,AW545629
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Molecular Weight
- 118.2 kDa
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NCBI Accession
- NP_033528
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Pathways
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
Target
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