Vitamin D Receptor Protein (VDR) (His tag)

Catalog No. ABIN7320044

Recombinant Vitamin D Receptor protein expressed in Baculovirus infected Insect Cells.

Quick Overview for Vitamin D Receptor Protein (VDR) (His tag) (ABIN7320044)

Target: Vitamin D Receptor (VDR)

Protein Type: Recombinant

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purity: > 86 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This Vitamin D Receptor protein is labelled with His tag.

Purpose: Recombinant Mouse VDR/NR1I1 Protein (His Tag)

Sequence: Met1-Ser422

Characteristics: A DNA sequence encoding the mouse VDR (P48281) (Met1-Ser422) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Vitamin D Receptor (VDR)

Alternative Name: VDR/NR1I1

Target Type: Chemical

Background:

Background: VDR (vitamin D(1,25- dihydroxyvitamin D3)receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1,25(OH)2D3) and induce expression of the 1,25(OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.

Synonym: Nr1i1

Molecular Weight: 49.2 kDa

UniProt: P48281

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway