Vaccinia Virus B18R (VACV B18R) protein (His tag)
Quick Overview for Vaccinia Virus B18R (VACV B18R) protein (His tag) (ABIN7198740)
Target
Protein Type
Origin
Source
Purity
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Purification tag / Conjugate
- His tag
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Purpose
- Recombinant Vaccinia Virus B18R / B19R Protein (His Tag)
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Sequence
- Met1-Glu351
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Characteristics
- A DNA sequence encoding the B18R (P25213) (Met1-Glu351) was expressed with a C-terminal polyhistidine tag.
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method
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Comment
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46 KDa
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Vaccinia Virus B18R (VACV B18R)
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Alternative Name
- VACV B18R
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Background
- B18R is a type I interferon (IFN)-binding protein, which is encoded by the B18R open reading frame in the WR (WesternReserve)strain of vaccinia virus. Itis alsoknownasB19RintheCopenhagenstrainofVaccinia. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. It has been shown that secreted B18R binds to uninfected and infected cells. It presents at the cell surface and protects cells from the antiviral state induced by IFN-alpha and IFN-beta. Binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.
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Molecular Weight
- 39.8KDa
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UniProt
- P25213
Target
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