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Vaccinia Virus B18R (VACV B18R) protein (His tag)

This Recombinant Vaccinia Virus B18R protein is produced in Baculovirus infected Insect Cells.
Catalog No. ABIN7198740
$1,154.40
Plus shipping costs $50.00
100 μg
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Quick Overview for Vaccinia Virus B18R (VACV B18R) protein (His tag) (ABIN7198740)

Target

Vaccinia Virus B18R (VACV B18R)

Protein Type

Recombinant

Origin

Vaccinia Virus (VACV)

Source

  • 2
Baculovirus infected Insect Cells

Purity

> 90 % as determined by SDS-PAGE
  • Purification tag / Conjugate

    His tag

    Purpose

    Recombinant Vaccinia Virus B18R / B19R Protein (His Tag)

    Sequence

    Met1-Glu351

    Characteristics

    A DNA sequence encoding the B18R (P25213) (Met1-Glu351) was expressed with a C-terminal polyhistidine tag.

    Endotoxin Level

    < 1.0 EU per μg of the protein as determined by the LAL method
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  • Comment

    46 KDa

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Please refer to the printed manual for detailed information.

    Buffer

    Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target

    Vaccinia Virus B18R (VACV B18R)

    Alternative Name

    VACV B18R

    Background

    B18R is a type I interferon (IFN)-binding protein, which is encoded by the B18R open reading frame in the WR (WesternReserve)strain of vaccinia virus. Itis alsoknownasB19RintheCopenhagenstrainofVaccinia. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. It has been shown that secreted B18R binds to uninfected and infected cells. It presents at the cell surface and protects cells from the antiviral state induced by IFN-alpha and IFN-beta. Binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.

    Molecular Weight

    39.8KDa

    UniProt

    P25213
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