SIGLEC10 Protein (Biotin)

Catalog No. ABIN7319878

Recombinant SIGLEC10 protein expressed in Human Cells.

Quick Overview for SIGLEC10 Protein (Biotin) (ABIN7319878)

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: >95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This SIGLEC10 protein is labelled with Biotin.

Purpose: Recombinant Human Siglec-10 (C-Fc-Avi) Biotinylated

Sequence: Met17--Thr546

Characteristics: Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 10 is produced by our Mammalian expression system and the target gene encoding Met17-Thr546 is expressed with a Fc, Avi tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Anti-Human Siglec10 mAb(Cat#NC035) at 1μg/ml (100 μl/well) can bind Biotinylated Human Siglec-10-Fc-Avi(Cat#CY47 ). The ED50 of Biotinylated Human Siglec-10-Fc-Avi(Cat#CY47 ) is 1.45 ng/ml.

Application Details

Comment:

100-130 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Alternative Name: Siglec-10

Background:

Background: Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

Synonym: SIGLEC10, MGC126774, PRO940, Siglec10, SLG2, sialic acid-binding Ig-like lectin 10, Siglec-10, siglec-like gene 2, Siglec-like protein 2, SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

Molecular Weight: 86.9 kDa

UniProt: Q96LC7