DDR2 Protein (GST tag,His tag)

Catalog No. ABIN7317114

This Recombinant DDR2 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for DDR2 Protein (GST tag,His tag) (ABIN7317114)

Target: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This DDR2 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human DDR2 Kinase/CD167b Protein (aa 422-855, His & GST Tag)(Active)

Sequence: Arg422-Glu855

Characteristics: A DNA sequence encoding the human DDR2 (Q16832) (Arg422-Glu855) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 8 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.

Application Details

Comment:

77 kDa

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Alternative Name: CD167b

Background:

Background: Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: CD167;MIG20a;NTRKR3;TKT;TYRO10

Molecular Weight: 77.1 kDa

UniProt: Q16832

Pathways: RTK Signaling