FZD8 Protein (Biotin,His-Avi Tag)

Catalog No. ABIN7319923

This Recombinant FZD8 protein is expressed in Human Cells.

Quick Overview for FZD8 Protein (Biotin,His-Avi Tag) (ABIN7319923)

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: >95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This FZD8 protein is labelled with Biotin,His-Avi Tag.

Purpose: Recombinant Human Frizzled-8 (C-6His-Avi) Biotinylated

Sequence: Ala28-Pro172

Characteristics: Biotinylated Recombinant Human Frizzled-8 is produced by our Mammalian expression system and the target gene encoding Ala28-Pro172 is expressed with a 6His, Avi tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Comment:

25-35 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Alternative Name: Frizzled-8

Background:

Background: Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.

Synonym: frizzled 8, frizzled family receptor 8, Frizzled8, Frizzled-8, FZ-8, FZD8, hFZ8

Molecular Weight: 19.1 kDa

UniProt: Q9H461

Pathways: WNT Signaling