Tryptophan Synthetase protein (His tag)

Catalog No. ABIN7320985

This Recombinant Tryptophan Synthetase protein is expressed in Escherichia coli (E. coli).

Quick Overview for Tryptophan Synthetase protein (His tag) (ABIN7320985)

Target: Tryptophan Synthetase

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant E.coli Tryptophan Synthase Protein (His Tag)

Sequence: Met1-Ser268&Thr2-Ile397

Characteristics: Recombinant E.coli Tryptophan synthase is produced by our E.coli expression system and the target gene encoding Met1-Ser268&Thr2-Ile397 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Comment:

28&40-50 kDa

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: Tryptophan Synthetase

Alternative Name: Tryptophan Synthase

Background:

Background: Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3'-phosphate (IGP) to give indole and D-glyceraldehyde 3'-phosphate (G3P). Indole is then transferred through a 25-? hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.

Synonym: Tryptophan synthetase, Tryptophan synthase

Molecular Weight: 72.5 kDa