APOA1 Protein (His tag)

Catalog No. ABIN7198959

Recombinant APOA1 protein expressed in Escherichia coli (E. coli).

Quick Overview for APOA1 Protein (His tag) (ABIN7198959)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This APOA1 protein is labelled with His tag.

Purpose: Recombinant Human Apolipoprotein A-I/ApoAI Protein (His Tag)

Sequence: Asp25-Gln267

Characteristics: A DNA sequence encoding the mature form of human APOA1 (P02647) (Asp25-Gln267) was expressed with a polyhistidine tag at the N-terminus.

Endotoxin Level: Please contact us for more information.

Application Details

Comment:

27-31 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 10 % Glycerol, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: Apolipoprotein A-I

Background:

Background: Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.

Synonym: Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1

Molecular Weight: 30.7 kDa

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism