Nuclease Protein
This Recombinant Nuclease protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN7271658
Quick Overview for Nuclease Protein (ABIN7271658)
Target
Nuclease
Protein Type
Recombinant
Origin
Serratia marcescens
Source
Escherichia coli (E. coli)
Purity
>95 % as determined by SDS-PAGE.
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Purpose
- GENIUS™ Nuclease, premium grade
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Sequence
- Met 1 - Asn 266
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Characteristics
- GENIUS™ Pro Nuclease is a tag free recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™ Pro Nuclease is a homodimer with monomer molecular masses about 30 kDa with an isoelectric point (pI) at pH 6.85. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'- phosphorylated oligonucleotides of 3-8 bases in length.
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Endotoxin Level
- Less than 0.01 EU per μg by the LAL method.
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Application Notes
- This protein carries no "tag".
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- 20 mM Tris, 20 mM NaCl, 2 mM MgCl2, pH 8.0
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Storage
- -80 °C
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Storage Comment
- -70°C
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- Nuclease
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Background
- Synonyms:GENIUS™Nuclease,BenzNuclease,BenzoNuclease,benzonase,Nuclease,Description:NA
Target
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