HLA-B*18:01 protein (SBP Tag)

Catalog No. ABIN7273192

This Recombinant protein is expressed in Mammalian Cells.

Quick Overview for HLA-B*18:01 protein (SBP Tag) (ABIN7273192)

Target: HLA-B*18:01

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Application: Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)

Purity: > 80 %

Product Details

Purification tag / Conjugate: SBP Tag

Purpose: The product is HLA-B*18:01 complexed with β2-microglobulin (b2m)

Sequence: heavy chain: GSHSMRYFYT AMSRPGRGEP RFISVGYVDD TQFVRFDSDA ASPREEPRAP WIEQEGPEYW DRNTQICKTN TQTYRESLRN LRGYYNQSEA GSHTLQRMYG CDVGPDGRLL RGHDQSAYDG KDYIALNEDL SSWTAADTAA QITQRKWEAA REAEQLRAYL EGLCVEWLRR YLENGKETLQ RADPPKTHVT HHPISDHEAT LRCWALGFYP AEITLTWQRD GEDQTQDTEL VETRPAGDRT FQKWAAVVVP SGEEQRYTCH VQHEGLPKPL TLRWEPSSQS TIPI
light chain: IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM
Sequences without tags. Tag location is at the discretion of the manufacturer.

Specificity: HLA-B*18:01 complexed with b2m, P01889 / P61769
HLA-B*B18:01 is most common in Nothern Italy and the Balkans with an average frequency of up to 17%. It reacts with anti-Bw6 antibodies.

Characteristics: HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, in complex with β2-microglobulin (HLA class I). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.

Purification: Chromatography

Endotoxin Level: Endotoxins have not been removed

Application Details

Application Notes: ELISA: 1 - 100 ng/mL

Comment:

sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies W6/32 and BBM.1 and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: TRIS based buffer, pH 8.0, Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 6 months

Target Details

Target: HLA-B*18:01

Background: Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class I (MHCI) molecule. Expressed on all cells, displays peptides to CD8-positive T cells. Involved in cellular immune response.

Molecular Weight: approx. 50 kDa (38 kDa heavy chain + 12 kDa b2m)

UniProt: P01889