TPBG Protein (AA 32-355) (Biotin,His-Avi Tag)

Catalog No. ABIN7273785

This Recombinant TPBG protein is produced in HEK-293 Cells.

Quick Overview for TPBG Protein (AA 32-355) (Biotin,His-Avi Tag) (ABIN7273785)

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 32-355

Purification tag / Conjugate: This TPBG protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human TPBG/5T4 Protein

Sequence: Ser32-Ser355

Characteristics: Recombinant Biotinylated Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser32-Ser355.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human TPBG, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test).,Biotinylated Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human TPBG is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Alternative Name: TPBG

Background: The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.

Molecular Weight: 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

UniProt: Q13641