CHIA Protein (AA 22-476) (His tag)
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- Target See all CHIA Proteins
- CHIA (Chitinase, Acidic (CHIA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-476
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CHIA protein is labelled with His tag.
- Purpose
- Human AMCase/CHIA Protein
- Sequence
- Tyr22-Ala476
- Characteristics
- Recombinant Human AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Ala476.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product CHIA Protein
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Chitinase, Acidic (CHIA) (AA 22-473) protein (His tag)
CHIA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chitinase, Acidic (CHIA) (AA 1-368) protein (GST tag)CHIA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Chitinase, Acidic (CHIA) (AA 22-476) protein (His tag)Crystallography grade CHIA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Chitinase, Acidic (CHIA) (AA 22-473) protein (His tag)Crystallography grade CHIA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Chitinase, Acidic (CHIA) protein (His tag)CHIA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CHIA (Chitinase, Acidic (CHIA))
- Alternative Name
- AMCase (CHIA Products)
- Synonyms
- chia-a Protein, AMCASE Protein, CHIT2 Protein, TSA1902 Protein, 2200003E03Rik Protein, AMCase Protein, YNL Protein, CHIA Protein, chioIIa Protein, fa55g10 Protein, wu:fa55g10 Protein, zgc:63792 Protein, acidic mammalian chitinase Protein, chitinase, acidic Protein, chitinase, acidic S homeolog Protein, chitinase-M31, acidic Protein, chitinase, acidic 1 Protein, chitinase, acidic.4 Protein, LOC703284 Protein, CHIA Protein, LOC100600050 Protein, chia.S Protein, CHIA-M31 Protein, Chia1 Protein, Chia Protein, chia.4 Protein
- Background
- Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. Despite that AMCase expression in the inflammation is under investigation, little is known regarding its regulation during macrophages' full maturation and polarization.
- Molecular Weight
- 51.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
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