CHIA Protein (AA 22-473) (His tag)
Quick Overview for CHIA Protein (AA 22-473) (His tag) (ABIN7273825)
Target
See all CHIA ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 22-473
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Purification tag / Conjugate
- This CHIA protein is labelled with His tag.
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Purpose
- Mouse AMCase/CHIA Protein
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Sequence
- Tyr22-Pro473
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Characteristics
- Recombinant Mouse AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Pro473.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Chitinase, Acidic (CHIA) (AA 22-476) protein (His tag)
CHIA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chitinase, Acidic (CHIA) (AA 1-368) protein (GST tag)CHIA Origin: Human Host: Wheat germ Recombinant ELISA, AA, AP, WB
Chitinase, Acidic (CHIA) protein (His tag)CHIA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CHIA (Chitinase, Acidic (CHIA))
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Alternative Name
- AMCase
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Background
- Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.
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Molecular Weight
- 51 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
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UniProt
- Q91XA9
Target
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