AMHRII (AA 18-144) (Active) Protein

Catalog No. ABIN7273826

Recombinant AMHRII protein expressed in HEK-293 Cells.

Quick Overview for AMHRII (AA 18-144) (Active) Protein (ABIN7273826)

Target: AMHRII

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-144

Purpose: Human AMHRII Protein

Sequence: Pro18-Ser144

Characteristics: Recombinant Human AMHRII Protein is expressed from HEK293 without tag.It contains Pro18-Ser144.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human AMHRII at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-AMHRII Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.,Human AMHRII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human AMHRII is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: AMHRII

Background: The aim of the current study was to explore whether anti-Müllerian hormone receptor II (AMHRII) genetic variants influence the hormonal profile and the ovarian response to standard gonadotropin stimulation of women undergoing medically assisted reproduction. Three hundred in vitro fertilization or intracytoplasmic sperm injection patients constituted the study population, while 300 women with at least one spontaneous pregnancy participated as controls.

Molecular Weight: 14.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

UniProt: Q16671