Annexin V Protein (AA 2-320)

Catalog No. ABIN7273831

This Recombinant Annexin V protein is expressed in Escherichia coli (E. coli).

Quick Overview for Annexin V Protein (AA 2-320) (ABIN7273831)

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 2-320

Purpose: Human Annexin V/ANXA5 Protein

Sequence: Ala2-Asp320

Characteristics: Recombinant Human Annexin V/ANXA5 Protein is expressed from E.coli without tag.It contains Ala2-Asp320.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Annexin V on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Annexin V is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 240 mM NaCl ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: Annexin V

Background: Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.

Molecular Weight: 35.9 kDa same as Bis-Tris PAGE result.

UniProt: P08758

Pathways: Apoptosis