ANGPTL4 Protein (AA 166-406) (His tag)

Catalog No. ABIN7273833

This Recombinant ANGPTL4 protein is produced in HEK-293 Cells.

Quick Overview for ANGPTL4 Protein (AA 166-406) (His tag) (ABIN7273833)

Target: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 166-406

Purification tag / Conjugate: This ANGPTL4 protein is labelled with His tag.

Purpose: Human ANGPTL4/Angiopoietin-like 4 Protein

Sequence: Pro166-Ser406

Characteristics: Recombinant Human ANGPTL4/Angiopoietin-like 4 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro166-Ser406.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human LILRB2, hFc Tag (Cat. LIL-HM2B2) captured on CM5 Chip via Protein A can bind Human ANGPTL4, His Tag with an affinity constant of 51.66 nM as determined in SPR assay (Biacore T200) (QC Test).,Human Angiopoietin-like 4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human LILRB2, hFc Tag (Cat. LIL-HM2B2) on ProA-Biosensor can bind Human ANGPTL4, His Tag with an affinity constant of 72.10 nM as determined in BLI assay (Gator® Prime).,The purity of Human Angiopoietin-like 4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Alternative Name: ANGPTL4

Background: Candidates for this common regulatory system include signals mediated by peroxisome proliferator-activated regulator and its response factor, angiopoietin-like 4. The expression and bioactivity of angiopoietin-like 4, an adipocytokine that was originally reported to have an angiogenic function, have been detected not only in the vascular system and adipose tissue but also in rheumatoid joints.

Molecular Weight: 28.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

UniProt: Q9BY76

Pathways: Regulation of Lipid Metabolism by PPARalpha