ANGPTL3 Protein (AA 17-219) (His-Avi Tag)

Catalog No. ABIN7273834

This Recombinant ANGPTL3 protein is expressed in HEK-293 Cells.

Quick Overview for ANGPTL3 Protein (AA 17-219) (His-Avi Tag) (ABIN7273834)

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 17-219

Purification tag / Conjugate: This ANGPTL3 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein

Sequence: Ser17-Lys219

Characteristics: Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser17-Lys219.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human LILRB2 captured on Protein A chip, can bind Human ANGPTL3, His Tag with an affinity constant of 17.6nM as determined in a SPR assay (Biacore T200).,Immobilized Human ANGPTL3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA (QC Test).,Human ANGPTL3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Alternative Name: ANGPTL3

Background: ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

Molecular Weight: 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.

UniProt: Q9Y5C1