Apelin Protein (APLN) (AA 23-77) (hFc-Avi Tag,Biotin)
Quick Overview for Apelin Protein (APLN) (AA 23-77) (hFc-Avi Tag,Biotin) (ABIN7273864)
Target
See all Apelin (APLN) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 23-77
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Purification tag / Conjugate
- This Apelin protein is labelled with hFc-Avi Tag,Biotin.
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Purpose
- Biotinylated Mouse APLN Protein
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Sequence
- Val23-Phe77
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Characteristics
- Recombinant Biotinylated Mouse APLN Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Val23-Phe77.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Biotinylated Mouse APLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Apelin (APLN) (AA 23-77) (Active) protein (hFc Tag)
APLN Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Apelin (APLN) (AA 1-77) protein (GST tag)APLN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Apelin (APLN) (AA 1-122) protein (GST tag)APLN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Apelin (APLN) (AA 23-77) protein (Fc Tag)APLN Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 0.2M Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- Apelin (APLN)
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Alternative Name
- APLN
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Background
- Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages.
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Molecular Weight
- 34.81 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9R0R4
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Pathways
- Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Feeding Behaviour
Target
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