APOE Protein (AA 19-317) (hFc Tag)

Catalog No. ABIN7273868

Recombinant APOE protein expressed in HEK-293 Cells.

Quick Overview for APOE Protein (AA 19-317) (hFc Tag) (ABIN7273868)

Target: APOE (Apolipoprotein E (APOE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 19-317

Purification tag / Conjugate: This APOE protein is labelled with hFc Tag.

Purpose: Human APOE4/Apolipoprotein E Protein

Sequence: Lys19-His317

Characteristics: Recombinant Human APOE4/Apolipoprotein E Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Lys19-His317.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human APOE4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 2 mM DTT, 5 mM CHAPS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE4

Background: Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).

Molecular Weight: 61.6 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Bis-Tris PAGE result.

Pathways: Regulation of Cell Size, Lipid Metabolism