APOE Protein (AA 19-311) (hFc Tag)

Catalog No. ABIN7273871

Recombinant APOE protein expressed in HEK-293 Cells.

Quick Overview for APOE Protein (AA 19-311) (hFc Tag) (ABIN7273871)

Target: APOE (Apolipoprotein E (APOE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-311

Purification tag / Conjugate: This APOE protein is labelled with hFc Tag.

Purpose: Mouse APOE/Apolipoprotein E Protein

Sequence: Glu19-Gln311

Characteristics: Recombinant Mouse APOE/Apolipoprotein E Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu19-Gln311.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse APOE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse APOE is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM PB, 150 mM NaCl ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE

Background: Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).

Molecular Weight: 60.7 kDa. Due to glycosylation, the protein migrates to 62-66 kDa based on Bis-Tris PAGE result.

UniProt: P08226

Pathways: Regulation of Cell Size, Lipid Metabolism