TNFSF13 Protein (AA 112-250, Trimer) (His-Avi-DYKDDDDK Tag)

Catalog No. ABIN7273872

This Recombinant TNFSF13 protein is produced in HEK-293 Cells.

Quick Overview for TNFSF13 Protein (AA 112-250, Trimer) (His-Avi-DYKDDDDK Tag) (ABIN7273872)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 112-250, Trimer

Purification tag / Conjugate: This TNFSF13 protein is labelled with His-Avi-DYKDDDDK Tag.

Purpose: Cynomolgus APRIL/TNFSF13 Trimer Protein

Sequence: Lys112-Leu250

Characteristics: Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus APRIL (Trimer) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus BCMA, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine ( pH 8.0). Normally 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL

Background: The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR.

Molecular Weight: 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5TJA1

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response