TNFSF13 Protein (AA 96-241) (Fc Tag)

Catalog No. ABIN7273875

Product Details

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Protein Type: Recombinant

Protein Characteristics: AA 96-241

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFSF13 protein is labelled with Fc Tag.

Purpose: Mouse APRIL/TNFSF13 Protein

Sequence: Ala96-Leu241

Characteristics: Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala96-Leu241.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse APRIL, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL (TNFSF13 Products)

Synonyms: APRIL Protein, CD256 Protein, TALL-2 Protein, TALL2 Protein, TRDL-1 Protein, ZTNF2 Protein, 2310026N09Rik Protein, April Protein, Tall2 Protein, Trdl1 Protein, SLAN Protein, ANP32B Protein, april Protein, tnf13 Protein, TNLG7B Protein, TNF superfamily member 13 Protein, tumor necrosis factor (ligand) superfamily, member 13 Protein, acidic leucine-rich nuclear phosphoprotein 32 family member B Protein, TNFSF13 Protein, Tnfsf13 Protein, LOC494442 Protein, tnfsf13 Protein

Background: The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.

Molecular Weight: 43.20 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.

UniProt: Q9D777

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response