TNFSF13 Protein (AA 112-250, Trimer) (His-Avi-DYKDDDDK Tag)

Catalog No. ABIN7273872

This Recombinant TNFSF13 protein is produced in HEK-293 Cells.

Quick Overview for TNFSF13 Protein (AA 112-250, Trimer) (His-Avi-DYKDDDDK Tag) (ABIN7273872)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 112-250, Trimer

Purification tag / Conjugate: This TNFSF13 protein is labelled with His-Avi-DYKDDDDK Tag.

Purpose: Non-biotinylated Cynomolgus APRIL/TNFSF13 Trimer Protein

Sequence: Lys112-Leu250

Characteristics: Recombinant Cynomolgus APRIL/TNFSF13 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Lys112-Leu250 trimer design.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus APRIL (Trimer), His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA (QC Test).,Cynomolgus APRIL Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Cynomolgus APRIL (Trimer), His Tag on Anti-His-Biosensor can bind Human TACI, hFc Tag with an affinity constant of 4.06 nM as determined in BLI assay (Gator® Prime).,Loaded Cynomolgus APRIL (Trimer), His-Avi Tag on Anti-His-Biosensor can bind Cynomolgus/Rhesus macaque BCMA, hFc Tag with an affinity constant of 1.59 nM as determined in BLI assay (Gator® Prime).,The purity of Cynomolgus APRIL Trimer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 200 mM L-arginine ( pH 8.0). Normally 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL

Background: The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFFR.

Molecular Weight: 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5TJA1

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response