TNFSF13 Protein (AA 96-241) (Fc Tag)
Quick Overview for TNFSF13 Protein (AA 96-241) (Fc Tag) (ABIN7273875)
Target
See all TNFSF13 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 96-241
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Purification tag / Conjugate
- This TNFSF13 protein is labelled with Fc Tag.
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Purpose
- Mouse APRIL/TNFSF13 Protein
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Sequence
- Ala96-Leu241
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Characteristics
- Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala96-Leu241.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Mouse APRIL, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 112-250), (Trimer) protein (His-DYKDDDDK Tag)
TNFSF13 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 112-250), (Trimer) protein (His-Avi-DYKDDDDK Tag)TNFSF13 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 1-247) protein (GST tag)TNFSF13 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 111-250) (Active) protein (His tag,DYKDDDDK Tag)TNFSF13 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-250) (Active) protein (Fc Tag,AVI tag,Biotin)TNFSF13 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) protein (Fc Tag)TNFSF13 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 112-250), (Trimer) protein (His-Avi-DYKDDDDK Tag,Biotin)TNFSF13 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-247) protein (T7 tag)TNFSF13 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
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Alternative Name
- APRIL
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Background
- The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
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Molecular Weight
- 43.20 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.
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UniProt
- Q9D777
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Target
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