ARTN Protein (AA 112-224) (hFc Tag)

Catalog No. ABIN7273877

Recombinant ARTN protein expressed in HEK-293 Cells.

Quick Overview for ARTN Protein (AA 112-224) (hFc Tag) (ABIN7273877)

Target: ARTN (Artemin (ARTN))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 112-224

Purification tag / Conjugate: This ARTN protein is labelled with hFc Tag.

Purpose: Mouse ARTN Protein

Sequence: Ala112-Gly224

Characteristics: Recombinant Mouse ARTN Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ala112-Gly224.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse ARTN, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse GFRA3, His Tag with the EC50 of 54.7ng/ml determined by ELISA (QC Test).,Mouse ARTN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 50 mM Glycine,150 mM NaCl ( pH 3.2). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Glycine,150 mM NaCl ( pH 3.2). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ARTN (Artemin (ARTN))

Alternative Name: ARTN

Background: Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.

Molecular Weight: 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.

UniProt: Q9Z0L2